Comparison of estradiol secretion in control versus HA and ECM- HA biomatrix. Estradiol secretion by embedded follicles was significantly different from the control . On Day 5, level of estradiol was highest in conditioned medium from control follicles as compared to HA and ECM-HA (a, P < 0.001). No difference was found between HA and ECM-HA. By Day 7 and 12 of culture estradiol production by ECM- HA embedded follicles far exceeded that in HA alone or in the control CT (b, P < 0.001). At both of these time points, follicles in the HA treatment group secreted less estradiol than controls (c, P < 0.001).