Table 1 Oligonucleotide primers and probes used for real-time RT-PCR analysis
Gene | GenBank accession no. | Sequence | Position (nucleotide no.) | |
|---|---|---|---|---|
ISG15 | NM_174366 | Forward primer | 5' GGGACCTGACGGTGAAGATG 3' | 67-86 |
| Â | Â | Reverse primer | 5' GAAAGCAGGCACATTGATCTTCT 3' | 160-182 |
| Â | Â | TaqMan probe | 5' TCCTGGTGCCTCTGAGGGACTCCAT 3' | 103-127 |
Mx2 | NM_173941 | Forward primer | 5' AAATCACCTACCGCAACATTACG 3' | 772-794 |
| Â | Â | Reverse primer | 5' GCCAAGTCCATTCCCAGCTA 3' | 853-872 |
| Â | Â | TaqMan probe | 5' ATGTTCTGGGCTCTCCGA 3' | 833-850 |
GAPDH | U85042 | Forward primer | 5' GGCACAGTCAAGGCAGAGAAC 3' | 238-258 |
| Â | Â | Reverse primer | 5' GGATCTCGCTCCTGGAAGATG 3' | 291-311 |
| Â | Â | TaqMan probe | 5' CATCAATGGAAAGGCCA 3' | 270-286 |