ISG15 and Mx2 mRNA expressions after intrauterine administration of IFNT. ISG15 (A) and Mx2 (C) mRNA expressions (mean ± S.E.M.) in PBMCs of cattle after intrauterine administration of IFNT (arrow; 0, 500, or 1000 μg); data are presented as relative fold change from the amount at 2 h before IFNT administration. *, P < 0.05 compared with value at 2 h before IFNT administration. Regression line of ISG15 (B) and Mx2 (D) mRNA expressions at 4 h after IFNT administration and concentration of IFNT administered into the uterus.