DNA damage to control and kisspeptin treated seminal vesicles. A) DNA ladder assay showing 180 bp bands of fragmented DNA, at 1 ng and 1 μg kisspeptin doses, from rat seminal vesicles. Faint bands were also visible in the 10 pg dose. Control (lane C) shows intact DNA. M = 100-bp DNA ladder. B) DNA fragmentation assay. Percentage of fragmented DNA relative to total DNA increased dose dependently with kisspeptin treatment. Values are expressed as mean ± SE. *P < 0.001.