Figure 4

The effects of insulin and/or testosterone on protein expression in T-HESCs cell cultures. Protein levels were assessed in cell exposed to 100 nM insulin (HI), 100 nM testosterone (HA), 100 nM insulin plus 100 nM testosterone (HI + HA), aand control cells. Representative Western Blot is shown below the bar graphs. Equal amounts of cell lysate were loaded in each lane, all the proteins were detected according to molecular mass: Munc18c (66 kDa) (A), PKCζ (68 kDa) (B), phospho-PKCζ (~68 kDa) (C) and Syntaxin-4 (34 kDa) (D). Band intensities were quantified by scanning densitometry and normalized to intensities observed for β-Actin. Results were expressed as relative units (RU), and the values are shown as mean ± SEM, * p < 0.05 between control and each hormonal treatment.