Figure 1

End-point RT-PCR analysis of transcripts for bovine BMP ligands and receptors in the peri-attachment conceptus (day 17 post-insemination), trophoblast cells (CT1) and endometrium (Endo.). After RT-PCR, reactions were electrophoresed in 1% [w/v] agarose containing ethidium bromide and amplicons were visualized using UV light. Three different conceptus and CT1 RNA preparations were used in each reaction but only a single endometrium RNA sample collected from cyclic cows at day 17 post-estrus was included. Panel A depicts BMP ligands and inhibitors. Panel B depicts BMP2/4 receptors. ACTB was included as a positive control.