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Table 1 Analytical recovery of defined concentrations of hCG-sLHCGR and sLHCGR spike into serum samples at three different known analyte concentrations

From: Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

Serum sample

Expected value Pmol/mL

Observed value Pmol/mL

Recovery %

Mean

SD

1

3.89

3.88

99.74

106.94

± 11.37

 

7.78

9.34

120.05

  
 

15.56

15.72

101.03

  

2

3.89

3.66

94.087

113.07

± 17.24

 

7.78

9.94

127.76

  
 

15.56

18.26

117.35

  

3

3.89

2.94

75.5787

90.53

± 12.99

 

7.78

7.71

99.1

  
 

15.56

15.08

96.915

  

4

3.89

3.2

82.262

82.34

± 2.7

 

7.78

6.62

85.09

  
 

15.56

12.4

79.692

  

5

3.89

3.86

99.229

90.66

± 7.5

 

7.78

6.63

85.219

  
 

15.56

13.62

87.532

  

6

3.89

4.92

126.48

115.1

± 9.85

 

7.78

8.5

109.25

  
 

15.56

17.05

109.58

  

7

3.98

2.55

64.07

52

± 11.9

 

7.97

4.13

51.82

  
 

15.95

6.43

40.313

  

8

3.98

2.9454

73.857

49.6

± 23.5

 

7.97

3.8396

48.139

  
 

15.95

4.2867

26.876

  

9

3.98

3.3188

83.386

74.7

± 39.4

 

7.97

8.692

109.06

  
 

15.95

5.0572

31.707

  
  1. Serum samples 1–6, hCG-sLHCGR; serum samples 7–9, sLHCGR.