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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

Figure 2

The specificity of sLHCGR ELISA assay. (a-c), demonstrates that LHCGR291 recombinant with C-terminal FLAG-tag specifically reacts with anti-FLAG and anti-LHCGR (LHR29) monoclonal antibodies; a) when plates coated with increasing amounts of LHR291 and CHO cell extracts were detected with anti-FLAG antibody and b) when plates coated with increasing amounts of LHR291 and CHO cell extracts were detected with serially diluted LHR29 antibodies or c), when plates coated with LHR29 antibody captured LHR291 in serially diluted extracts and was detected by anti-FLAG antibody: (d-f), the concentration-dependent reactivity of the LHCGR protein is shown where d) LHR29-PG732-HRP, e) anti-FLAG-LHR29-HRP and f) LHR29-anti-FLAG-HRP combinations were used as capture-detection antibodies respectively in ELISA assays specifically detecting LHCGR291 recombinant protein.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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