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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: potential diagnostics in first trimester pregnancy screening for stillbirth, Down’s syndrome, preterm delivery and preeclampsia

Figure 1

Epitope mapping of LHR29 monoclonal antibody. a) Three recombinant proteins containing 229, 291 or 318 amino acid residues of the LHCGR extracellular domain and C-terminal FLAG-epitope were expressed in CHO cells as described [12]; b), recombinant as well as mock transfected extracts (no DNA) were resolved in SDS-PAGE, blotted and probed with anti-FLAG, LHR29 and PG732 mono- and polyclonal antibodies respectively; c), two monoclonal antibodies (LHR74 and LHR29) and one polyclonal (PG732) were used in western blot analysis of proteins extracted from placental extracts at 12 wks of gestation.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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