Figure 3

In situ hybridization of BMP-4 mRNA in ovaries of adult cycling rats. Brighfield (A, C, D, E, G, H, J, K, M, O) and Darkfield (B, F, I, L, N). A, B: Sections from P 1000 h ovaries (4X). Positive OSE, and T; weakly positive CL; negative preantral follicles (arrow heads); C: Positive SC () at P 1000 h (40X); D: Positive endothelial cells of large sinusoid (40X); E, F: Secondary follicle at P 1000 h (20X) showing positive cells (arrow heads) at periphery of T. G: High power (40X) of E showing positive theca interstitial cells (TIC) (arrows) in outer half of TI and positive cells (arrows) in the TE. H, I: Dominant follicle at P 2000 h showing positive theca (10X); J: Higher magnification (40X) of H showing positive TE and adjacent TIC in the dominant follicle. K, L: Older CL-I (10X) at E 1000 h of the next cycle showing positive TE (arrow heads); M, N: CL-I at P 1000 h (20X); showing positive TE (arrow head) and OSE only over the new CL; O: Higher magnification of CL-I showing positive OSE and underlying TE (40X).