Figure 7
(A, B) Western blots with 100 zona free germinal vesicle intact oocytes (lane 1) and 100 zona free unfertilized oviductal oocytes (lane 2) probed with 10 μg/ml of LCA and (C) western blot with 50 zona free germinal vesicle intact oocytes (lane 1) and 50 zona free unfertilized oviductal oocytes (lane 2) probed with 10 μg/ml of LCA preincubate with α-D-methyl-mannopyranoside followed by HRP-streptavidin. (A) Same LCA blotting pattern was found in six separate experiments. (B) Same LCA blotting pattern was found in two separate experiments. (C) This control was run separately three times.