Figure 2

Confocal laser scanning micrographs of 30 μm to 70 μm mouse germinal vesicle intact oocytes. All oocytes were zona free and double labeled with ABL₂ (B, E, H, K, and N) and LCA (A, D, G J, and M). Some images of ABL₂ and LCA labeled oocytes were merged (C, F, I, L, and O). Various sizes of germinal vesicle intact oocytes are shown as follows: 30 μm (A–C), 40 μm (D–F), 50 μm (G–I), 60 μm (J–L), and 70 μm (M–O). Fig. 2L insert shows a large LCA-stained perinuclear area with a subregion that was stained with both LCA and ABL₂ (yellow at the tip of the arrow).