Effects of ICI 182, 780 on the expression of ERα in the rat efferent ductules. In the control rats (A, B, E, F, I, J), ERα was expressed in the nuclei of ciliated (c) and nonciliated cells in the efferent ductules epithelium. Cytoplasmic staining was observed in the ciliated cells (c) at the proximal tubules (A, E, I and insert in E) and in both cell types in the distal tubules (EDd) (B, F, J). After 7 days of ICI treatment (C, D) a slight decrease in ERα was observed along the efferent ductules. On day 45 (G, H), ERα expression was greatly decreased with the treatment. On day 150 (K, L), the ERα expression was further decreased. Insert in A represents the negative control. Bar in A = 50 μm.