Figure 2

Mean (± sed) progesterone production by granulosa cells after (A) 48 hours and (B) 144 hours and mean (± sed) oestradiol production by granulosa cells after (C) 48 hours and (D) 144 hours in serum free culture in the presence (dark bars) and absence (light bars) of oocyte conditioned medium (OCM), 1 ng/ml pFSH and either an optimal dose of Long R3 IGF-1 (100 ng/ml) or no Long R3 IGF-1. Values are from 3 independent cultures, each treatment having 4 replicates. There was a significant effect of treatment (P < 0.001) on progesterone production at both time points. Bars with no common superscripts are significantly different (P < 0.05).