Figure 3

In situ hybridization localization of SPAM1 transcripts in human corpus epididymal epithelium. Histological sections were hybridized with either an anti-sense (A) or sense (B) SPAM1 RNA probe. SPAM1 transcripts localized by immunolabeling appear as a purple stain in the epithelium of (A). This stain is absent from the negative control (B) which shows only the neutral red counterstaining and which was incubated for the same period of time as A) in the developing solution.