LHR immunoreactivity and resident macrophages in the female pelvic floor. A) Vaginal smooth muscle (Vag SM): cytoplasmic (full arrowhead) and nuclear LHR immunoreactivity (white arrowhead). B) Vaginal connective tissue (Vag ct): fibroblast type cells (white arrowhead), rounded mesenchymal cells (full arrowhead) and vessels (red arrowhead). C) Vaginal epithelium (Vag Ep): basal (stem) cells (b), parabasal cells (full arrowhead) and intermediate cells (yellow and green arrowheads). Superficial layer (middle inset): nuclear staining (red arrowhead) and diminution of staining in the surface cells (white arrowhead). D) Levator ani fascia (LF): principal cells (fibroblasts; white arrowhead) and rounded mesenchymal cells (full arrowhead). E) Levator ani muscle (see also F for detail): mesenchymal cells among muscle fibers (full arrowhead) and muscle cell nuclei (white arrowheads). CD68 staining in vaginal connective tissue (G), levator fascia (H) and levator muscle (I). Insets indicated as ctr show control staining. No hematoxylin counterstain except panels G–I. Bar in I for all panels except E.