Table 1 Gene references, primer sequences, hybridization temperature during PCR and theoretical length of amplified fragments
Equine gene | Genbank references | primer (5' to 3') | strand | Temperature of hybridization | length of amplified fragments |
|---|---|---|---|---|---|
Glyceraldehyde-3-Phosphate Deshydrogenase (gapdh) | af157626 | GTTTGTGATGGGCGTGAACC TTGGCAGCACCAGTAGGAGC | s a-s | 62°C | 255 bp |
Interleukin-1α | u92480 | GCCAATGATCCAGAAGAAGG CAGTTTTCCCTGCTTTGTGG | s a-s | 60°C | 450 bp |
Interleukin-1β | u92481 | GGTGTTCTGCATGAGCTTTG TTTCTGCTTGAGAGGTGCTG | s a-s | 60°C | 266 bp |
Interleukin-1ra | u92482 | TGCAAGATGCAAGCCTTCAG TGGAGGTAGAACTTGGTGAC | s a-s | 60°C | 425 bp |
Interleukin-1R1 | ab020338 | TGATCTGCAATGTCACTGGC AATCCCTGTACCAAAGCACC | s a-s | 60°C | 372 bp |
Interleukin-1R2 | ab033415 | ATGCTCTCTGGATTTTGCCG TACGAGTAAACGAGGGGGTC | s a-s | 62°C | 311 bp |