Figure 3

Immunofluorescent analysis of integrin α_vβ₃ in cryostat cross-sections of endometrium. (A) Immunoreactivity in sections from heifers treated with control solution, interferon-tau (IFN-τ), estrogen or ICI 182, 780. Diffuse staining was present in the dense stroma, low reactivity was observed in the glands (GE) and luminal epithelium (LE). Note the strong reactivity of intercaruncular subepithelial stromal cells (arrows) in sections from estrogen and ICI 182, 780 treated animals. CARS – caruncular stroma; ICS – intercaruncular stroma. Magnification was 100X. B) Least squares means ± standard error of signal intensity of integrin α_vβ₃ in the subepithelial stroma (SES) by treatment (3 animals × 4 replicate experiments per treatment). Intensity was scored on a six point scale (0-negative, 1-very weak, 2-weak but clearly positive, 3-intermediate, 4-high, and 5-very high) and the data were subjected to ANOVA and the Tukey-Kramer multiple comparison of means to determine effects of treatment in comparison to the control. Asterisks indicate means that are significantly different from the control (P < 0.05).