Figure 3

Dose-dependent stimulation of ActRI mRNA levels by activin-A. Cells were incubated for 12 h in the absence or presence of activin-A (0.1–30 ng /ml). cPCR was performed to measure ActRI mRNA levels. The data were pooled from 3 separate experiments and expressed as a percentage of control value (mean ± SEM). Data were normalized for β-actin mRNA levels measured by PCR. *(p < 0.05) or **(p < 0.01) versus control.