Figure 4

Lectin binding to whole oocytes and embryos. FITC-labeled lectins were used to analyze carbohydrates on the surface of VE and FE . Ten to twenty eggs were incubated with the lectin, washed, and observed under a fluorescence-inverted microscope. Controls were carried out as for lectin blots. Results are shown for ConA (a-d), PNA (e-h), HAA (i-l) and WGA (m-p). Micrograph exposure times were the same in all cases, except for j, which was over-exposed to record the observations described. "Vis" (q-t) indicates eggs photographed under visible light. Images are representative of four independent experiments.