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Table 1 Primers for FSH and LH receptors and Connexin-43.

From: Responsiveness of bovine cumulus-oocyte-complexes (COC) to porcine and recombinant human FSH, and the effect of COC quality on gonadotropin receptor and Cx43 marker gene mRNAs during maturation in vitro

 

5' (5'-3')

3' (5'-3')

Primer binding temp. (°C)

Product size (bp)

FSHr

CAACCTGCTATACATCGACC

GAGCAAGTCACATCAACCAC

53

723 537

LHr

AGAGTGAACTGAGTGGCTGG

CAACACGGCAATGAGAGTAG

53

533

Cx43

TGGCTGCTCCTCACCAACG/CGC

AGGTCATCAGGCCGAGGT/CCTG

58

334