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Table 1 The EFFECT of TUNICAMYCIN on 3H-FUCOSE INCORPORATION in cumulus, ooplasm and PVS, as observed on semi-thin sections

From: The extracellular matrix of porcine mature oocytes: Origin, composition and presumptive roles

    Labeling
Oocyte stage at onset
of experiment
Duration
of culture (hr)
Culture
medium (1)
Cumulus
cells
(2)
ooplasm
PVS
GV (0 hr post hCG) 8   +++ + ++
  8 T +++   +
  36    ++ +++
  36 T   + +
LD (20 hr post hCG) 8   +++ ++ ++
  8 T +++ + +
  20    ++ ++
  20 T   + +
  1. (1) The modified TC 199 was supplemented with 3H-Fucose in the presence (T) or absence of Tunicamycin; (2) To evaluate labeling on semi-thin sections the following scale was used : ++++ very intense, +++intense, ++medium,+ just over background