The extracellular matrix of porcine mature oocytes: Origin, composition and presumptive roles

Table 1 The EFFECT of TUNICAMYCIN on ³H-FUCOSE INCORPORATION in cumulus, ooplasm and PVS, as observed on semi-thin sections

			Labeling
Oocyte stage at onset of experiment	Duration of culture (hr)	Culture medium (1)	Cumulus cells	(2) ooplasm	PVS
GV (0 hr post hCG)	8		+++	+	++
	8	T	+++		+
	36			++	+++
	36	T		+	+
LD (20 hr post hCG)	8		+++	++	++
	8	T	+++	+	+
	20			++	++
	20	T		+	+

(1) The modified TC 199 was supplemented with ³H-Fucose in the presence (T) or absence of Tunicamycin; (2) To evaluate labeling on semi-thin sections the following scale was used : ++++ very intense, +++intense, ++medium,+ just over background

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