Figure 3

Western analysis of RARα and RXRβ expression in bovine cumulus-granulosa cells. Lanes 1 and 2 represent immunoreactive RARα and RXRβ protein, respectively, in bovine cumulus-granulosa cell nuclear extracts. The relative molecular mass of both RARα and RXRβ proteins was determined by molecular mass markers. The relative molecular mass of both RARα and RXRβ proteins recognized by the respective polyclonal antibodies was very close to the relative molecular mass described by the manufacturer of the antibody.