Advancements in fertility preservation strategies for pediatric male cancer patients: a review of cryopreservation and transplantation of immature testicular tissue

Sung, Zih-Yi; Liao, Yong-Qi; Hou, Jung-Hsiu; Lai, Hong-Hsien; Weng, Sung-Ming; Jao, Hai-Wei; Lu, Buo-Jia; Chen, Chi-Huang

doi:10.1186/s12958-024-01219-5

Table 3 Summary of xenologous testicular tissue grafting in mammalian species

From: Advancements in fertility preservation strategies for pediatric male cancer patients: a review of cryopreservation and transplantation of immature testicular tissue

Reference	Testicular Tissue Donor	Testicular Tissue Recipient	Grafting Size	Grafting Site	Cryopreserved or Fresh	Outcome
Shinohara et al. [10] 2002	Prepubertal Rabbits	ICR nude mice	8 to 16 mm³	Testicular parenchyma	Frozen/Thawed and fresh	Spermatogenesis occurrence: 74% in fresh and 90% in frozen transplants
Shinohara et al. [10] 2002	Prepubertal Rabbits	ICR nude mice	8 to 16 mm³	Testicular parenchyma	Frozen/Thawed and fresh	One male offspring was produced
Honaramooz et al. [49] 2002	Prepubertal ICR or B6C3F1 mice, pigs and goats	Immunodeficient mice	0.5 to 1 mm³	Back skin	Frozen/Thawed and fresh	Graft recovery: more than 60%
Honaramooz et al. [49] 2002	Prepubertal ICR or B6C3F1 mice, pigs and goats	Immunodeficient mice	0.5 to 1 mm³	Back skin	Frozen/Thawed and fresh	Sperm were all viable and functional from testis grafts of all three species into mice
Snedaker et al. [50] 2004	Prepubertal domestic kittens	Immunodeficient mice	0.5 to 1 mm³	Back skin	Frozen/Thawed	Graft recovery: 45.5%
Snedaker et al. [50] 2004	Prepubertal domestic kittens	Immunodeficient mice	0.5 to 1 mm³	Back skin	Frozen/Thawed	Complete spermatogenesis occurred after transplantation
Rathi et al. [97] 2005	Prepubertal cattle	Immunodeficient mice	1 to 2 mm in diameter	Back skin	Fresh	Graft recovery: 80%
Rathi et al. [97] 2005	Prepubertal cattle	Immunodeficient mice	1 to 2 mm in diameter	Back skin	Fresh	In most grafts, spermatogenesis was arrested at the pachytene spermatocyte stage
Rathi et al. [52] 2006	Prepubertal, pubertal and adult horses	Immunodeficient mice	1 to 2 mm in diameter	Back skin	Fresh	Graft recovery: 65%
Rathi et al. [52] 2006	Prepubertal, pubertal and adult horses	Immunodeficient mice	1 to 2 mm in diameter	Back skin	Fresh	The pubertal group leaded to the most complete spermatogenesis
Arregui et al. [53] 2008	Prepubertal sheep	Immunodeficient mice	1 mm³	Back skin	Fresh	Graft recovery: 95.7%
Arregui et al. [53] 2008	Prepubertal sheep	Immunodeficient mice	1 mm³	Back skin	Fresh	Complete spermatogenesis occurred after transplantation
Abrishami et al. [24] 2010	Prepubertal, pubertal and adult dogs	Immunodeficient mice	2 mm³	Back skin	Fresh	Graft recovery: 79.2%
Abrishami et al. [24] 2010	Prepubertal, pubertal and adult dogs	Immunodeficient mice	2 mm³	Back skin	Fresh	Prepubertal and pubertal testis xenografts maintained complete spermatogenesis
Reddy et al. [55] 2012	Prepubertal buffalos	Immunodeficient mice	Weighing 8-10 mg	Back skin	Fresh	Graft recovery: 89.4% after 12 weeks and 78.3% after 24 weeks
Reddy et al. [55] 2012	Prepubertal buffalos	Immunodeficient mice	Weighing 8-10 mg	Back skin	Fresh	Complete spermatogenesis occurred only in intact recipients
Ntemou et al. [56] 2019	Prepubertal marmosets	Swiss Nu/Nu mice	0.8 to 1 mm³	(1) Back skin	Fresh	Graft recovery after 4 months: 21% from back skin and 50% from intratesticular
Ntemou et al. [56] 2019	Prepubertal marmosets	Swiss Nu/Nu mice	0.8 to 1 mm³	(2) Intratesticular	Fresh

This highlights the frequent use of the back skin of immunodeficient mice as a graft site. ICR, Institute of Cancer Research

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ISSN: 1477-7827

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