Fig. 4

EnSCs reversed cisplatin-induced ferroptosis in vivo and in vitro. A-B Western blotting was performed to detect the GPX4 protein expression levels, which were quantified. C qRT-PCR was conducted to assess the mRNA expression levels of GPX4. D Animal models establishment process. E Measurement of serum total antioxidant capacity in mice. F Ovarian morphology and follicle numbers were presented by H & E staining. G GPX4 expression levels in ovarian tissue were displayed by immunohistochemistry (n = 5 mice per group). Scale bars in the HE staining represent 500 μm, while in the IHC staining represent 100 μm. The results were expressed as mean ± SEM (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, * versus control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001, ^# versus cis (10 µM cisplatin) group)