Fig. 4

The expression level of LHCGR in ESCs. A The ESCs treated with hCG (0.1IU/mL, 72 h) was analyzed by immunofluorescence to determine the subcellular localization and protein expression level of LHCGR (red). The nuclei were stained with DAPI (blue). Scale, 50 μm and 20 μm. B The qRT-PCR analysis of LHCGR gene in ESCs after hCG treatment. *P < 0.05, **P < 0.01 (Student’s t-test). C Western blot analysis of LHCGR protein in ESCs after hCG treatment. *P < 0.05, **P < 0.01 (Student’s t-test)