Fig. 6

Downregulation of PHBs partly impairs decidualization. The PHBs protein expression level on day 2 or day 10 after transfection of T-HESCs with 10 nM siPHB1 (A), 10 nM siPHB2 (B), respectively, was analyzed by western blot. Densitometric analysis was performed with imagej and values were normalized to β-actin. The PRL mRNA expression changes in T-HESCs with (red line) and without (black line) induction was determined by qPCR and normalized to HPRT1 as a reference gene (C). The PRL mRNA expression changes in T-HESCs transfected with 10 nM siPHB1 (D), 10 nM siPHB2 (E) upon decidualization induction were determined by qPCR. Results are shown as the mean ± SEM from three biological replicates. Statistical analysis was performed by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001