Fig. 2

A rise-to-decline expression pattern of PGRMC1 was revealed by in vitro decidualization. A Schematic representation of in vitro decidualization system. The cellular morphology changes of T-HESCs on day 0 and day 10 were imaged with microscopy in bright filed (B) or immunofluorescence staining (C). PGRMC1 was stained by Alexa Fluor-488 (green), and the nucleus was stained by DAPI in blue. Scale bar: 200 µm. The mRNA expression levels of PRL in T-HESCs were analyzed with qRT-PCR when cells were cultured with MPA/cAMP (red line) for decidualization or DMSO (black line) as control (D). The dynamic changes of PGRMC1 protein expression from 1 to 14 days induction and non-induction control (on Day 14) were measured by western blot (E) and the bar plot with the relative densitometric analysis of the corresponding PGRMC1 protein level (p value calculation based on ‘D0’) (F). β-actin was used as a loading control. The mRNA expression levels of PGRMC1 in T-HESCs during decidualization (G). Results are shown as the mean ± SEM from three biological replicates. Statistical analysis was performed by two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001