Fig. 4

Changes in METTL3 expression were involved in embryo implantation by regulating cellular decidualization in vivo. a Flowchart of animal experiments. b Mouse models of endometriosis were established and the ectopic lesions are shown. c Number of blastocysts in normal pregnant mice (n = 5) and mice with endometriosis (n = 8) was counted (the left panel, scale bar = 1 cm). d Either the METTL3 inhibitor (STM2457) or the control solvent dimethyl sulfoxide (DMSO) were injected into the horn of the uterus on either side of the mice with endometriosis (n = 10). The number of blastocysts per side of the uterus was counted (the left panel, scale bar = 1 cm). e Protein levels of FOXO1 on the DMSO-injected and STM2457-injected sides of the mouse uterus were detected by IHC staining on day 8 (scale bar = 100 μm or scale bar = 50 μm). All experiments were repeated in triplicate or quadruplicate. Data with error bars are presented to indicate the mean ± SEM values. ***P < 0.001, ****P < 0.0001