Fig. 2

METTL3 expression was downregulated during the decidualization of primary endometrial stromal cells (ESCs) in vitro. These cells were treated with medroxyprogesterone acetate (MPA) and 8-Bromo-cyclic adenosine monophosphate (8-Br-cAMP) for the decidual treatment for 0, 2, 4, and 6 d. The mRNA (a, b) and protein (c, d) levels of prolactin (PRL) and insulin-like growth factor-binding protein 1 (IGFBP1) were detected using qRT-PCR and western blot analysis, respectively. e, f mRNA and protein levels of forkhead box O1 (FOXO1) were detected by qRT-PCR and western blot analysis, respectively. g Immunofluorescence (IF) staining was performed to detect FOXO1 levels (scale bar = 100 μm). h IF staining was performed to detect morphological changes in cells after decidual treatment (scale bar = 50 μm). i, j Protein levels of METTL3 were detected by western blot analysis and IF staining (scale bar = 100 μm). k Dot blot assays were performed to detect m6A changes in cells after decidual treatment. Methylene blue staining was used as a control. l Protein levels of METTL3 in ESCs derived from normal (ND) and endometriosis (ED) groups, both of which received decidual treatments. All experiments were repeated in triplicate or quadruplicate. The blots in this figure are cropped (please refer to supplementary files for details). Data with error bars are presented as the mean ± SEM values. **P < 0.01, ***P < 0.001, ****P < 0.0001