Table 5 Comparison of advantages and disadvantages between laser-assisted and mechanical immobilized sperm
From: Applications of laser technology in the manipulation of human spermatozoa
Procedures | Principle | Advantage | Disadvantage | References |
|---|---|---|---|---|
Mechanical immobilization | Immobilization is performed by touching the tail of the sperm with ICSI pipette. | • No need for additional equipment • Easy to standardize and adapt in clinical scenarios | • Resulting in sperm membrane damages • Require PVP to facilitate sperm capture | |
Laser-assisted immobilization | Sperm is immobilized with 1.48 μm wavelength diode laser. | • Simple and time-saving • Retaining membrane integrity • Noncontact • Avoiding the harmful effects of PVP on sperm and embryos | • Need for laser equipment • Literature insufficiency on the percentage of live birth |