Fig. 6
From: Decreased HAT1 expression in granulosa cells disturbs oocyte meiosis during mouse ovarian aging
Depletion of HAT1 decreases FoxO1 nuclear location and downregulates AREG expression in GCs. (A)-(B) Western blot analysis of the expression of AREG in the CTL and si-HAT1 treated KGN cells. The experiments were repeated three times independently with similar results. (C) Five possible binding sites of the AREG promoter region and FoxO1. (D)-(F) Western blot analysis of cytosolic and nuclear fractions in the control and si-HAT1 group. The experiments were repeated three times independently with similar results. (G) Dual-luciferase reporter genes assay of AREG promoter and FoxO1. Data are shown as the mean ± SEM, ns, no significance, *P < 0.05, **P < 0.01, Student’s t test