Fig. 5

Depletion of HAT1 increases GCs apoptosis and downregulates acetylated FoxO1 expression. (A)-(B) Western blot analysis of the expression of caspase 3, cleaved-caspase 3, Bax, and Bcl2 in the CTL and si-HAT1 treated KGN cells. The samples used to examine both the efficiency of si-HAT1 knockdown (Fig. 2E) and the level of apoptosis (Fig. 5A) were from the same batch of KGN cells, which leads to the same loading control. The experiments were repeated three times independently with similar results. (C) Representative micrographs of the CTL and si-HAT1 treated KGN cells stained with JC-1. Scale bar: 100 μm. (D) Ratios of red: green JC-1 fluorescence in the CTL and si-HAT1 groups. The experiments were repeated three times independently with similar results. (E)-(F) Western blot analysis of the expression of FoxO1, p-FoxO1, and Ac-FoxO1 in the control and si-HAT1 group. The experiments were repeated three times independently with similar results. Data are shown as the mean ± SEM, ns, no significance, *P < 0.05, **P < 0.01, Student’s t test