Fig. 4

HAT1 inhibition causes differential gene expression in oocytes. (A) The rate of PBE at different times from 6 to 14 h in oocytes from the CTL and AA groups. The data are shown with at least three independent experiments at each time point. (B) Representative micrographs of the PBE oocytes at 10 h culture time in the CTL and AA groups. Left, Scale bar: 200 μm. Right, Scale bar: 100 μm. (C) Flowchart overview of mice single oocyte RNA sequencing in the CTL and AA groups. (D) PCA of the transcriptome of RNA sequencing data in oocytes collected from the CTL and AA groups. (E) Heatmap showing differential gene expression in the CTL and AA groups. (F)-(G) Representative GO enrichment analysis of signal pathways of the downregulated pathways (left) and upregulated pathways (right) between the CTL and AA groups