Fig. 4

FN-Binding assays of peri-implantation blastocysts treated with Wnt proteins and different inhibitors. (A) Microscopy images of FN-binding assays realized on 3.5 dpc blastocysts cultured in control L cells CM, Wnt5a CM and Wnt7a CM with the addition of different inhibitors. Binding activity was visualized in green, and nuclei were stained with iodure propidium (red). (B) Microscopy images of FN-binding assays realized on 3.5 dpc blastocysts cultured in control L cells CM, Wnt5a CM and Wnt7a CM and with the CamKII inhibitor KN93. (C) Fluorescent intensity of the green signal was quantified and used to evaluate the binding activity of 3.5 dpc blastocysts cultured in control L cells CM, Wnt5a CM and Wnt7a CM and with the CamKII inhibitor KN93. **2way ANOVA, P < 0.01 and Dunnett’s multiple comparisons test