Fig. 3

FN-Binding assays of peri-implantation blastocysts in vitro and in vivo. (A) Microscopy images of FN-binding assays realized on 3.5 dpc blastocysts cultured in KSOM alone, uterin fluid (UF) ± sFRP2 ± Wnt7a CM. Binding activity was visualized in green and nuclei were stained with iodure propidium (red). (B) Fluorescent intensity of the green signal was quantified and used to evaluate the binding activity of 3.5 dpc blastocysts cultured in KSOM alone, uterin fluid (UF) ± sFRP2 ± Wnt7a CM. * ANOVA test, P < 0.05 and Holm-Šídák’s multiple comparisons test. (C) Microscopy images of FN-binding assays realized on 3.5 dpc blastocysts cultured in vitro in KSOM or transplanted into the uterus with KSOM ± sFRP2 ± Wnt7a CM. Binding activity was visualized in green, and nuclei were stained with iodure propidium (red). (D) Fluorescent intensity of the green signal was quantified and used to evaluate the binding activity of 3.5 dpc blastocysts in vitro in KSOM or transplanted into the uterus with KSOM ± sFRP2 ± Wnt7a CM. * ANOVA test, P < 0.05 and Holm-Šídák’s multiple comparisons test