Fig. 2

FN-Binding assays of peri-implantation blastocysts treated with Wnt proteins. (A) Microscopy images of FN-binding assays realized on 3.5 dpc blastocysts cultured in control L cells CM, Wnt5a CM and Wnt7a CM for varying incubation times. Binding activity was visualized in green, and nuclei were stained with iodure propidium (red). (B) Fluorescent intensity of the green signal was quantified and used to evaluate the binding activity of 3.5 dpc blastocysts cultured in control L cells CM, Wnt5a CM and Wnt7a CM for varying incubation times. * 2 way ANOVA test, P < 0.05 and Šídák’s multiple comparisons test. Different letters indicate a significant difference, while the same letter indicates a non-significant difference