Fig. 1

Expression of Itgα5β1 in peri-implantation blastocysts treated with Wnt proteins. (A) Microscopy images of 3.5 dpc blastocysts cultured in KSOM alone or with control CM (L cells), Wnt5a CM and Wnt7a CM and stained with (total Itgα5β1) or without (surface Itgα5β1) permeabilization with anti-Itgα5β1 antibody (green). Nuclei were stained with iodure propidium (red). (B) The ratio between the green signal (Itgα5β1) and red signal (iodure propidium) was used to evaluate the total Itgα5β1 expression in 3.5 dpc blastocysts cultured for 48 h in KSOM alone, control CM, Wnt5a CM and Wnt7a CM and stained with permeabilization. (C) The fluorescent intensity of the green ratio (Itgα5β1) was used to evaluate the surface Itgα5β1 expression in 3.5 dpc blastocysts cultured for 48 h in KSOM alone, control CM, Wnt5a CM and Wnt7a CM and stained without permeabilization. * ANOVA test, P < 0.05