Fig. 3

Transcription factors universally regulating endometrial progression and function and their expression throughout the menstrual cycle. A Regulatory network of TFs forming relationships with numerous gene lists (2–18). TFs targeting specific gene lists were placed in the center of the network. CTCF stood out as the only TF that targeted genes belonging to 18/19 (95%) different signatures. B In-silico analysis of CTCF and GATA6 expression throughout the menstrual cycle. Significant changes between phases are marked with asterisks (**FDR ≤ 0.01, ***FDR ≤ 0.001). C RT-qPCR validation. Bar plot shows the relative mRNA expression of CTCF and GATA6 in endometrial biopsies collected throughout the menstrual cycle. Data is presented as a mean ± SD. Significant differences of CTCF (*FDR < 0.05) and GATA6 (***FDR < 0.001) expression were observed between proliferative/early-secretory (PF/ESE) and mid-secretory/late-secretory phases (MSE/LSE). Abbreviations in (B and C): PF, Proliferative; ESE, early-secretory; MSE, mid-secretory; LSE, late-secretory; SD, standard deviation