Fig. 5

Absolute transcript quantification of key placental components involved in placental 5-HT clearance using digital droplet PCR analysis. Expression of SERT, OCT3, MAO-A, and MRP2 was analyzed in the placental tissues used in the perfusion and explant studies, and in primary trophoblast cells isolated from human term placenta. The results are reported in numbers of transcripts per ng of transcribed RNA. Data are presented as individual biological replicates with horizontal lines indicating median values; n = 5