Fig. 3

5-HT clearance by isolated placental explants and primary trophoblast cells. The capacity of tissue samples and cells to take up 5-HT and metabolize it to 5-HIAA was evaluated at 5-HT concentrations of 100 nM (A, D) and 100 µM (B, E) over a period of 6 h. This process was concentration-dependent, with the 100 nM substrate concentration giving significantly higher 5-HIAA/5-HT ratios at 4-hour incubation time (C, F). Data are presented for individual biological replicates with lines connecting median values or as medians with interquartile ranges; n = 5. Statistical significance was evaluated using the Mann-Whitney test. * p ≤ 0.05, ** p ≤ 0.01