Fig. 3
From: Paternal Expressed Gene 10 (PEG10) is decreased in early-onset preeclampsia
PEG10 gene expression reduces with cytotrophoblast differentiation. First-trimester human cytotrophoblast stem cells (hTSCs) were differentiated into syncytiotrophoblasts (ST) over 96 h (A-D). Effective syncytialisation was confirmed by a decrease in trophoblast markers, TEAD4 (A) and CDH2 (B) and an increase in ST marker, SDC1 (C). PEG10 mRNA expression significantly decreased by 96 h in ST cells (D). hTSCs were differentiated into extravillous trophoblast (EVT) cells across 144 h as shown by an increase in EVT marker, HLAG (E). PEG10 mRNA expression was significantly decreased after 144 h of EVT differentiation (F). Data expressed as a median with interquartile range. Individual symbols represent n = 5 experimental repeats. P** < 0.01, P*** < 0.001