Fig. 2

Localisation of PEG10 protein and mRNA within the placenta. Immunohistochemistry (IHC) staining of human placental villi shows PEG10 protein (brown) localised to cytotrophoblasts in both control and early-onset preeclampsia tissue (A). Semi-quantitative, blinded assessment of IHC staining indicated reduced PEG10 protein in early-onset preeclampsia placentas relative to controls (C). In situ hybridisation shows PEG10 (blue) and PECAM-1 (pink) mRNA expression. PEG10 expression localised to cytotrophoblasts in both control and early-onset preeclampsia tissues while PECAM-1 marked placental vasculature (B). No staining observed in respective isotype controls (minimised image). Figures were captured at 40 × magnification and are representative of 5 experimental repeats. Data expressed as mean \(\pm\) SEM. Individual symbols represent individual scores and P* < 0.05