Fig. 6

DUXAP8 can affect the TFPI2 expression through recruiting EZH2 in the of trophoblasts nucleus. A The FISH assay was also used to detect the DUXAP8 location the in HTR-8/SVneo cells. The results showed that the DUXAP8 was in both the nucleus and the cytoplasm. B The cytoplasmic and nuclear RNA isolation assay was conducted to detect the DUXAP8 location in cells. The results showed that the DUXAP8 is in the nucleus of cells mostly. The GAPDH and U1 were used as controls. C The RIP was conducted to investigate the possible protein bound to the DUXAP8. The results showed that EZH2, SUZ12, and LSD1 were bound to the DUXAP8. D The ChIP was conducted to find the relationship between EZH2, H3K27me3, and TFPI2. The results showed EZH2 and H3K27me3 enrichment in the promoter region of TFPI2. The enrichment was decreased as cells were transfected with the si-DUXAP8. Data results were expressed as mean ± SEM, *P < 0.05, **P < 0.01