Fig. 5

Gene expression profiling. A The RNA transcriptome sequencing analysis was performed to analyze gene expression profiling in the HTR-8/SVneo cells following the DUXAP8 knockdown. B GO analysis showed different gene expression correlated to the cell growth in cells transfected with the si-DUXAP8. C The qPCR assay was conducted to certify the genes related to the DUXAP8. D The western blot was conducted to test the protein expression of TFPI2 in the HTR-8/SVneo and JAR cells with the downregulated DUXAP8. The GAPDH was used as a control. Data results were expressed as mean ± SEM, *P < 0.05, **P < 0.01