Fig. 1

DUXAP8 expression in placental tissues and cell lines. A The DUXAP8 expression in placental tissues was tested by the qPCR and was normalized in GAPDH. In each group, the DUXAP8 expression in the PE placenta tissue was compared to the normal control. The data were represented as log2 fold changes (PE/normal, shown as − ΔΔCT) and defined as “ < 0” for underexpression and “ > 0” for overexpression. B The DUXAP8 relative expression was presented as the fold change in the PE placental samples compared with the corresponding normal tissues (n = 33). C The DUXAP8 expression in cell lines was tested by the qPCR. D The knockdown DUXAP8 expression was detected by the qPCR after transfecting the JAR and HTR-8/SVneo with a synthesized si-DUXAP8-1#, 2#, 3#. The knockdown DUXAP8 percentage was shown in the figure compared to si-NC. The interference efficiency of #1 reached 85%. E The DUXAP8 overexpression was detected by the qPCR after transfecting the JAR and HTR-8/SVneo with a synthesized plasmid pcDNA-DUXAP8 (pDUXAP8). The DUXAP8 relative expression compared to the pcDNA was shown in the Fig. E. The multiples of overexpression efficiency reached 400. Data results were expressed as mean ± SEM, *P < 0.05, **P < 0.01