Fig. 6

Hematoxylin and eosin staining (HE stain) and immunohistochemical staining of CD90 (upper row), CD105 (middle row), and Sushi domain containing 2 (SUSD2) (lower row) in the neonatal endometria/myometria and adult endometria/myometria of panel A. Arrows indicate CD90- or CD105-stained cells in respective endometria or myometria. No SUSD2-stained cells were identified in neonatal and adult endometria or myometria. In addition to adult endometria, neonatal brain tissue (inset) and lung tissue (inset) were used as positive controls for CD90, CD105, and SUSD2. Respective slides of negative controls (inset) without the use of first antibody are shown on the extreme right slides of panel (A). The quantitative analysis of CD90- and CD105-stained cells in endometria (E), myometria (M) and combined endometria + myometria (E + M) and significance between groups for each marker are shown on the panel (B, C, D, E). The details of quantitative analysis are described in methods. The boxes represent the interquartile ranges and horizontal lines in the boxes represent median values. Scale bar = 50 and 100 μm