Fig. 3

Hematoxylin and eosin staining (HE stain) and immunohistochemical staining of PRL, IGFBP1, Gd-A, and α-SMA in neonatal and adult endometria are shown in panel (A). The quantitative analysis of PRL-, IGFBP1-, Gd-A-, and α-SMA-stained cells and significance between groups for each marker is shown on the right panel (B). The details of quantitative analysis are described in methods. A slide of negative controls without use of first antibody is shown on the extreme right row of panel (A). In addition to adult endometria, decidual tissue and pituitary gland were used as positive controls (inset). The boxes represent the interquartile ranges and horizontal lines in the boxes represent median values. PRL, prolactin-decidual marker; IGFBP1, insulin growth factor binding protein 1- decidual marker; Gd-A, Glycodelin-A (also named placentral protein 14)-pre-decidual marker; α-SMA, alpha-smooth muscle actin-pre-decidual marker. Scale bar = 50 and 100 μm