Fig. 2

Hematoxylin and eosin staining (HE stain) and immunohistochemical staining of EpCAM, CD10, ER, PGR, Ki-67, and CD31 in neonatal and adult endometria are shown in panel (A). The quantitative analysis of EpCAM-, CD10-, ER-, PGR-, Ki-67-, and CD31-stained cells and significance between groups for each marker is shown on the right panel (B). The details of quantitative analysis of each marker are described in methods. A slide of negative controls without use of first antibody is shown on the extreme right row of panel (A). The boxes represent the interquartile ranges and horizontal lines in the boxes represent median values. EpCAM, marker of glandular epithelial cells; CD10, marker of stromal cells; ER, estrogen receptor; PGR, progesterone receptor; Ki-67, marker of cell proliferation; CD31, marker of vascular cells. Scale bar = 50 and 100 μm