Fig. 4

Regulation of BCL9 by miR-15a-5p in EcESCs. A Venn diagram of the target genes of miR-15a-5p predicted by Starbase, TargetScan and miRDB databases. B RT-qPCR to detect BCL9 expression in 30 EMS eutopic and ectopic tissues, and 20 normal endometrial tissues. C TargetScan online prediction of miR-15a-5p. D Dual luciferase reporter gene assay to verify the target binding of miR-15a-5p to BCL9. E RT-qPCR to detect the expression of miR-15a-5p and BCL9 after overexpression or silencing of miR-15a-5p. F Western blot analysis to detect the protein expression of BCL9 after overexpression or silencing of miR-15a-5p, F Western blot analysis to detect the protein expression of BCL9 after overexpression or silencing of miR-15a-5p. * p < 0.05 indicates the comparison between two groups; ** p < 0.01 indicates the comparison between two groups; ns p > 0.05 indicates the comparison between two groups